异搏定对小鼠肝癌腹水瘤细胞系PI-PLC活性的影响

本文采用615近交系小鼠肝癌腹水瘤H_(ca)-F_(25)/CL-A_2细胞,测定钙拮抗剂异搏定作用前后磷脂酰肌醇特异的磷脂酶C(PI-PLC)活性的变化,并与同样处理的瘤株内钙恒稳的有关指标相比较。结果表明经异搏定处理后的A_2细胞PI-PLC活性显著降低,并与钙恒稳的指标基本呈现相应的平行变化趋势。提示PI-PLC可能参与肿瘤细胞内钙恒稳的变化过程,异搏定的作用可能与磷脂酰肌醇信号传导系统相关。

The Effect of Verapamil on Activities of PI-PLC from an Ascites Hepatoma Cell Clone

H_(ca)-F_(25)/CL-A_2, a type of ascites hepatoma cell clones of 615 inbreeding strain mice was employed and the activities of phosphatidylinositol-specific phospholipase C (PI-PLC) were measured before and after treated with verapamil, and compared with some indexes of intracellular calcium homeostasis. The results showed that PI-PLC activities were decreased in the tumor cells after treated with verapamil. These changes occurred simultaneously with those changes indexes of calcium homeostasis. The results suggested that PI-PLC involved in changing the process of intracellular calcium homeostasis, and the effect of verapamil was closely associated with the phosphoinositide signaling pathway in the tumor cells.