Assessing genetic diversity in a sugarcane germplasm collection using an automated AFLP analysis |
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| Authors: | P Besse G Taylor B Carroll N Berding D Burner CL McIntyre |
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| Institution: | (1) Commonwealth Scientific and Industrial Research Organisation (CSIRO), Tropical Agriculture, 306 Carmody Road, St Lucia, Qld, 4067, Australia;(2) Bureau of Sugar Experiment Stations (BSES), PO Box 86, 50 Meiers Rd, Indooroopilly, Qld, 4068, Australia;(3) Departments of Agriculture and Biochemistry, University of Queensland, Brisbane, Qld, 4072, Australia;(4) BSES, Meringa Experimental Station, Gordonvale, Qld, 4865, Australia;(5) USDA‐ARS, Sugarcane Research Unit, Houma, LA 70361‐ 0470, USA |
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| Abstract: | An assessment of genetic diversity within and between Saccharum, Old World Erianthus sect. Ripidium, and North American E.giganteus
(S.giganteum) was conducted using Amplified Fragment Length Polymorphism (AFLPTM) markers. An automated gel scoring system (GelComparTM) was successfully used to analyse the complex AFLP patterns obtained in sugarcane and its relatives. Similarity coefficient
calculations and clustering revealed a genetic structure for Saccharum and Erianthus sect. Ripidium that was identical to
the one previously obtained using other molecular marker types, showing the appropriateness of AFLP markers and the associated
automated analysis in assessing genetic diversity in sugarcane. A genetic structure that correlated with cytotype (2n=30,
60, 90) was revealed within the North American species, E. giganteus (S.giganteum). Complex relationships among Saccharum,
Erianthus sect. Ripidium, and North American E.giganteus were revealed and are discussed in the light of a similar study which
involved RAPD markers.
This revised version was published online in July 2006 with corrections to the Cover Date. |
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| Keywords: | AFLPTM automated analysis diversity GelComparTM sugarcane |
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