Genetic transformation ofRhododendron byAgrobacterium tumefaciens |
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| Authors: | Kei-ichiro Ueno Yutaka Fukunaga Ken-ichi Arisumi |
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| Institution: | (1) Kagoshima Biotechnology Institute, 893-16 Kushira, Kagoshima, Japan;(2) Takii Agricultural Experimental Station, 520-32 Kousei, Shiga, Japan;(3) Faculty of Agriculture, Kagoshima University, Kagoshima 890, Japan |
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| Abstract: | Summary TransgenicRhododendron plants were obtained byAgrobacterium tumefaciens-mediated gene transfer.A. tumefaciens harboring a binary vector that contained the chimeric neomycin phosphotransferase II (NPTII) and (3-glucuronidase (GUS) genes was co-cultivated with stem and leaf segments fromRhododendron tissues culturedin vitro. Adventitious buds were fonned and shoots were regenerated on kanamycin selection medium 3-4 months after inoculation. Integration of the NPTII and the GUS genes was confirmed by polymerase chain reaction (PCR) and by Southern hybridization analyses. Histochemical GUS assay showed that the inserted gene was expressed in all tissues with the cauliflower mosaic virus (CaMV) 35S promoter. This transformation procedure has the potential to expand the range of genetic variation inRhododendron. |
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