An Rts1-derivative plasmid conferring UV sensitivity on Escherichia coli host |
| |
Authors: | Y Terawaki Y Kobayashi H Matsumoto Y Kamio |
| |
Affiliation: | Department of Bacteriology, Shinshu University School of Medicine, Matsumoto, 390 Japan |
| |
Abstract: | A deletion mutant was isolated from a kanamycin resistance R plasmid Rtsl. This mutant plasmid, pTW20, was found to enhance the lethal effect of UV irradiation on host, especially at 42°C. A cloning experiment with pTW20 DNA demonstrated that the gene, , being responsible for the UV sensitivity was located on the kanamycin resistance gene containing H1 fragment of pTW20. This fragment conferred a sensitivity to methyl methane sulfonate on its host along with the sensitivity to UV, suggesting that a reapir process of the host chromosome is impaired by the presence of . |
| |
Keywords: | |
本文献已被 ScienceDirect 等数据库收录! |
|