In vitro screening for resistance against Septoria nodorum blotch in wheat |
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Authors: | W Wicki M Messmer M Winzeler P Stamp J E Schmid |
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Institution: | (1) Swiss Federal institute of Technology Zürich, Department of Plant Sciences, ETH-Zentrum, CH-8092 Zürich, Switzerland e-mail: juerg.schmid@ipw.agrl.ethz.ch Fax: ++41-52-354-91-19, CH;(2) Swiss Federal Research Station for Agroecology and Agriculture, CH-8046 Zürich, Switzerland, CH |
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Abstract: | This study was carried out to develop an in vitro test for the identification of genotypes resistant to Septoria nodorum blotch. The basis for this project was a previous study in which a crude extract of S. nodorum was used as a selective agent (Keller et al. 1994). It was possible to distinguish resistant and susceptible cultivars in
an in vitro test with zygotic embryos. In our project we wanted to test whether this in vitro test can also be used to detect
resistant and susceptible genotypes in early segregating populations. Specific crosses between eight winter wheat lines showing
contrasting resistance reaction for S. nodorum blotch on leaves and ears were made. The resistance level of both leaf and ear was evaluated after artificial inoculation
in the field for the parental lines, the F1 progenies, as well as for segregating F3 and F4 populations. In addition, this plant material was tested in vitro using methods similar to those described by Keller et al.
(1994), i.e. culturing immature zygotic embryos and mature seeds on selective media. A good agreement between in vitro screening
and field resistance on the ear was found for the parental lines, the F1 and F4 generation but not for the F3 generations. This leads to the conclusion that the in vitro screening might be integrated into wheat breeding programs. Populations
showing a high susceptibility to the pathogen metabolites in vitro could be discarded. Another promising implementation for
wheat breeding would be the screening of advanced breeding material or candidate partners in a crossing program for resistance
on the ear. However, the in vitro screening is not precise enough to select single plants in early segregating populations.
Received: 18 January 1999 / Accepted: 30 April 1999 |
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Keywords: | In vitro selection Septoria nodorum Triticum aestivum L Crude extract Toxin resistance |
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