The kinetics of binding of oxygen and carbon monoxide to Gastrophilus haemoglobin |
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| Authors: | Charles F Phelps Eraldo Antonini Maurizio Brunori and George Kellett |
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| Institution: | Department of Biochemistry, University of Bristol Medical School, University Walk, Bristol BS8 1TD, U.K., and Centro di Biologia Moleculare, Istituto di Chimica Biologica, University of Rome, Rome, Italy |
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| Abstract: | The dimeric haemoglobin in the tracheal cells of the Gastrophilus larva was extracted and purified, and the spectral properties of its oxy- and carbon monoxide adducts are recorded. In dilute solutions the kinetic parameters of binding of oxygen and carbon monoxide were determined. In solutions between 0.1 and 50mum for oxygen k(on) is 1x10(7)m(-1).s(-1) and k(off) is 1s(-1); for carbon monoxide l(on) is 6.5x10(5)m(-1).s(-1) and l(off) is 0.14s(-1). These values are in agreement with previous equilibrium results on oxygen binding and carbon monoxide/oxygen partition. These results are discussed and compared with the known values for other monomeric protohaem proteins. |
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