Utility of the FLP-FRT recombination system for genetic manipulation of rice |
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Authors: | Parthiban Radhakrishnan Vibha Srivastava |
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Institution: | Department of Crop, Soil and Environmental Sciences, University of Arkansas, Fayetteville, AR, 72701, USA. pradhak@uark.edu |
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Abstract: | To develop an FLP-FRT recombination system- (derived from 2 mu plasmid of Saccharomyces cerevisiae) based marker gene removal application for rice, we introduced the gene for FLP recombinase, under the control of the maize ubiquitin-1 promoter, into the rice genome. FLP activity was monitored in callus and regenerated plants by an assay based on the deletion of the FRT-flanked DNA fragment, leading to the activation of the beta-glucuronidase gene. FLP activity was detected both in the callus and leaves of some of the transgenic lines. Based on our comparison of the recombination efficiency of the FLP-FRT system expressed in the transgenic lines with that of the widely used Cre-lox system (derived from bacteriophage P1), we suggest that the FLP-FRT system is a useful tool for the genetic manipulation of rice. |
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