Pseudovibrio denitrificans strain Z143-1, a heptylprodigiosin-producing bacterium isolated from a Philippine tunicate |
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Authors: | Sertan-de Guzman Alice A Predicala Rey Z Bernardo Evelyn B Neilan Brett A Elardo Sheila P Mangalindan Gina C Tasdemir Deniz Ireland Chris M Barraquio Wilfredo L Concepcion Gisela P |
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Affiliation: | Marine Science Institute, University of the Philippines, Diliman, Quezon City, Philippines;;School of Biotechnology and Biomolecular Science, University of New South Wales, Sydney, NSW, Australia;;Department of Medicinal Chemistry, University of Utah, Salt Lake City, UT, USA;and;Institute of Biology, University of the Philippines, Diliman, Quezon City, Philippines |
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Abstract: | Microbial isolate Z143-1 found to be associated with an unidentified tunicate was characterized due to its significant antimicrobial activity. Z143-1 is similar to Pseudovibrio ascidiaceicola and Pseudovibrio denitrificans in morphological, physiological and biochemical characteristics, except for its ability to ferment glucose and produce a characteristic red pigment. Fatty acid methyl ester analysis revealed a predominance of the fatty acid 18:1 omega7c at 80.55%, at levels slightly lower than the Pseudovibrio denitrificans type strain DN34(T) (87.7%). The mol% G+C of Z143-1 is 54.02, relatively higher than the Pseudovibrio denitrificans type strain DN34(T) and Pseudovibrio ascidiaceicola with mol% G+C of 51.7 and 51.4, respectively. However, phylogenetic analysis of the 16S rRNA gene sequence of Z143-1 showed 100% similarity with the Pseudovibrio denitrificans type strain DN34(T). In this study, the bacterium Z143-1 is reported as a new strain of Pseudovibrio denitrificans. While there is no report of a secondary metabolite for Pseudovibrio denitrificans, Z143-1 produces the red pigment heptylprodigiosin, also known as 16-methyl-15-heptyl-prodiginine, which shows anti-Staphylococcus aureus activity. |
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Keywords: | Pseudovibrio denitrificans heptylprodigiosin 16-methyl-15-heptyl-prodiginine |
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