| 响应面法优化酿酒酵母产油脂条件 |
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| 引用本文： | 杨实权,张喜成,刘军锋,王芳,谭天伟,邓利.响应面法优化酿酒酵母产油脂条件[J].微生物学通报,2010,37(1):0091-0095. |
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| 作者姓名： | 杨实权 张喜成 刘军锋 王芳 谭天伟 邓利 |
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| 作者单位： | 北京化工大学生命科学与技术学院北京市生物加工过程重点实验室生物炼制教育部工程研究中心,北京,100029 |
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| 基金项目： | 国家973计划项目(No. 2007CB707804, 2007CB714304); 国家自然科学基金项目(No. 20876012, 20876011); 北京市自然基金(No. 2071002); 北京市教育委员会共建项目专项资助 |
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| 摘 要： | 运用响应面法对酿酒酵母(Saccharomyces cerevisiae)产油脂以及发酵条件优化进行了研究。首先根据单因素实验结果,利用Plackett-Burman设计对影响其产油脂相关因素进行评估并筛选出具有显著效应的3个因素:柠檬酸,CaCl2和初始pH值。接着用最陡爬坡试验逼近以上3个因子的最大响应区域后,采用Box-Behnken设计以及响应面分析法,确定其优化后发酵条件为(w/v):葡萄糖15%,蛋白胨0.2%,酵母浸粉0.4%,柠檬酸0.471%,MgSO4·7H2O0.1%,ZnSO4·7H2O0.2%,CaCl20.025%,FeSO4·7H2O0.005%,初始pH值为6.74,180r/min,30°C培养96h。优化后的油脂产率(干重)达到14.55%,比在种子培养基中油脂产率4.76%提高了2倍左右。
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| 关 键 词： | 酿酒酵母 油脂 响应面法 优化 |
Response Surface Methodology to Optimize Lipid Production Conditions by Saccharomyces cerevisiae |
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| YANG Shi-Quan,ZHANG Xi-Cheng,LIU Jun-Feng,WANG Fang,TAN Tian-Wei and DENG Li.Response Surface Methodology to Optimize Lipid Production Conditions by Saccharomyces cerevisiae[J].Microbiology,2010,37(1):0091-0095. |
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| Authors: | YANG Shi-Quan ZHANG Xi-Cheng LIU Jun-Feng WANG Fang TAN Tian-Wei and DENG Li |
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| Institution: | College of Life Science and Technology, Beijing University of Chemical Technology, The Key Laboratory of Bioprocess of Beijing, The Biorefinery Research and Engineering Center of the Ministry of Education of China, Beijing 100029, China;College of Life Science and Technology, Beijing University of Chemical Technology, The Key Laboratory of Bioprocess of Beijing, The Biorefinery Research and Engineering Center of the Ministry of Education of China, Beijing 100029, China;College of Life Science and Technology, Beijing University of Chemical Technology, The Key Laboratory of Bioprocess of Beijing, The Biorefinery Research and Engineering Center of the Ministry of Education of China, Beijing 100029, China;College of Life Science and Technology, Beijing University of Chemical Technology, The Key Laboratory of Bioprocess of Beijing, The Biorefinery Research and Engineering Center of the Ministry of Education of China, Beijing 100029, China;College of Life Science and Technology, Beijing University of Chemical Technology, The Key Laboratory of Bioprocess of Beijing, The Biorefinery Research and Engineering Center of the Ministry of Education of China, Beijing 100029, China;College of Life Science and Technology, Beijing University of Chemical Technology, The Key Laboratory of Bioprocess of Beijing, The Biorefinery Research and Engineering Center of the Ministry of Education of China, Beijing 100029, China |
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| Abstract: | Response surface methodology (RSM) was employed to optimize culture medium for lipid production of Saccharomyces cerevisiae. Firstly, according to the results of mono-factors experiment, a Plackett-Burman design was used to investigate the effects of different factors in the ferment medium, three statistically significant factors are:citric acid, CaCl2, initial pH value, and then steepest ascent procedures was employed to define optimal response region for these three factors. Finally, Box-Behnken was employed to design. The results of experiments was analyzed by RSM with Minitab15.0 soft. The optimal lipid production medium is: glucose 15%, peptone 0.2%, yeast extraction 0.4%, citric acid 0.471%, MgSO4?7H2O 0.1%, ZnSO4?7H2O 0.2%, CaCl2 0.025%, FeSO4?7H2O 0.005%, initial pH value 6.74, 180 r/min, incubme in 30?C for 96 h. Lipid production (dry weight) after optimization is increased from 4.76% to 14.55%. The yield of lipid is increased by about 3 times. |
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| Keywords: | Saccharomyces cerevisiae Lipid Response surface methodology Optimization |
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