亚油酸铂靶向脂质体抗肿瘤特性的研究

用超声波制备了内部包裹亚油酸铂，表面有抗人乳腺癌单克隆抗体ＭｃＡｂＧｐ－１Ｄ８的亚油酸铂靶向脂质体和亚油酸铂非靶向脂质体，研究了这些脂质体绎腹部注射到荷瘤裸鼠之后的组织分布和抑瘤效果，实验结果表明，靶向脂质体亚油酸铂在肿瘤组织的含量明显高于游离亚油酸铂组；在肾，肝，肺，脾，心脏等器官中，前者的含量比后者有所降低，分别在接种癌细胞后６天，１２天和２４天，按６ｍｇ／ｋｇ的剂量分别注射ＰＢＳ，游离亚油酸

A STUDY ON THE ANTICANCEROUS CHARACTERISTICS OF THE TRAGETED LIPOSOMES CONTAINING LINOLEIC PLATINUM

Linoleic platinum-containing untargeted and targetal liposomes learing antihuman Breast cancer cell monoclonal antibody Me Ah Gp-1D8 on their surface are prepared by sonication,The tissue distribution of linoleic platinum encapsulated liposomes and its efficacy of tumor inhibit after injection i.p. tumor-boring nude mice as compared with that of free linoleic platinum were investigated. The result indicated that the content of linoleic platinum of targeted liposomes in the tumor thenes was apparently higher than that of free linoleic platinum graup.However, the conent of the former was lower than the latter in heart, liver, lung, spleen and kidney. Free linoleic platinum or targeted liposomes were injected i.p. into nude mice (4mg/Kg body weight) at day 6,12,and 24, respectively, and the data were obtained at 40 day after inoculation of breast cell. The result shows that linoleic platinum targeted liposomes has advantage over free linoleic platinum in some respects such as the amount of survived animals, the ralio of tumor occurrence and the rate of tumor growth. The interaction of linoleic platinum targeted liposome with tumor cell membranes has been investigated by mothod of MSL spin Labeling. In the presence of linoleic platinum the ratio W/S of breast cancer cell membranes and S180 Cell membranes labeled with MLS were changed. The result indicates that linoleic platinum can react with mercapto groups and change the conformations of membranes proteins of tumor cells.