The yeast Rgd1p is a GTPase activating protein of the Rho3 and rho4 proteins. |
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Authors: | F Doignon C Weinachter O Roumanie M Crouzet |
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Affiliation: | Laboratoire de Biologie Moléculaire et de Séquen?age, UPR CNRS 9026, BP Box 64, 146 rue Léo Saignat, 33076, Bordeaux, France. |
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Abstract: | The RGD1 gene, identified during sequencing of the Saccharomyces cerevisiae genome, encodes a protein with a Rho-GTPase activating protein (GAP) domain at the carboxy-terminal end. The Rgd1 protein showed two-hybrid interactions with the activated forms of Rho2p, Rho3p and Rho4p. Using in vitro assays, we demonstrated that Rgd1p stimulated the GTPase activity of both Rho3p and Rho4p; no stimulation was observed on Rho2p. In addition, the rho3Deltargd1Delta double mutant exhibited a dramatic growth defect compared to the single mutants, suggesting that Rgd1p has a GAP activity in vivo. The present study allowed the identification of the first GAP of Rho3p and Rho4p. |
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