Recovery of proteins and amino acids from reverse micelles by dehydration with molecular sieves |
| |
Authors: | Gupta R B Han C J Johnston K P |
| |
Institution: | Department of Chemical Engineering, The University of Texas at Austin Austin, Texas 78712-1062. |
| |
Abstract: | A new method is presented to precipitate proteins and amino acids from reverse micelles by dehydrating the micelles with molecular sieves. Nearly complete precipitation is demonstrated for alpha-chymotrypsin, cytochromec, and trytophan from 2-ethylhexyl sodium sulfosuccinate (AOT)/isooctane/water reverse micelle solutions. The products precipitate as a solid powder, which is relatively free of surfactant. The method does not require any manipulation of pH, ionic strength, temperature, pressure, or solvent composition, and is applicable over a broad range of these properties. This general approach is compared with other techniques. This general approach is compared with other techniques for the recovery of biomolecules from reverse micelles. (c) 1994 John Wiley & Sons, Inc. |
| |
Keywords: | reverse micelles protein precipitation amino acid precipitation |
本文献已被 PubMed 等数据库收录! |
|