| ABI 1700芯片分析系统在基因差异表达研究中的应用 |
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| 引用本文： | 丁大鹏,马文丽,石嵘,周珏宇,郭秋野,郑文岭.ABI 1700芯片分析系统在基因差异表达研究中的应用[J].生命科学研究,2007,11(2):134-138. |
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| 作者姓名： | 丁大鹏 马文丽 石嵘 周珏宇 郭秋野 郑文岭 |
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| 作者单位： | 南方医科大学,基因工程研究所,中国广东,广州,510515;华南基因组中心,中国广东,广州,510830 |
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| 摘 要： | 应用化学发光法标记技术分别对正常和临床慢性髓细胞性白血病（chronic myelogenous leukemia,CML）病人骨髓单个核细胞的RNA进行标记,然后与ABI的人全基因组表达谱芯片杂交,对于杂交后所得到的荧光信号数据,应用1700芯片分析系统对其进行生物信息学分析.实验结果表明,ABI1700芯片分析系统可以对基因芯片杂交后得到的差异表达基因进行疾病学分类和生物功能分类分析,同时还发现与CML相关的差异表达基因75个,因此ABI1700芯片分析系统在芯片研究领域中具有重要的应用价值.
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| 关 键 词： | ABI 1700芯片分析系统 差异表达基因 慢性髓细胞性白血病 |
| 文章编号： | 1007-7847（2007）02-0134-05 |
| 修稿时间： | 2006-12-282007-04-10 |
Application of 1700 Bioanalyzer in the Study of Gene Differential Expression |
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| DING Da-peng,MA Wen-li,SHI Rong,ZHOU Jue-yu,GUO Qiu-ye,ZHENG Wen-ling.Application of 1700 Bioanalyzer in the Study of Gene Differential Expression[J].Life Science Research,2007,11(2):134-138. |
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| Authors: | DING Da-peng MA Wen-li SHI Rong ZHOU Jue-yu GUO Qiu-ye ZHENG Wen-ling |
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| Institution: | 1. Institute of Genetic Enginee ring, Nanfang Medical University, Guangzhou 510515, Guangdong, China; 2. Genome Center of Southern China, Guangzhou 510830, Guangdong, China |
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| Abstract: | The RNAs extracted from one chronic myelogenous leukemia(CML) patient and one control sample were labelled with chemiluminescence method. Differences of gene expression profiles were examined by ABI 1700 Bioanalyzer.The physiology-disease categories and biological processes of differential expressed genes were determined with analysis of ABI 1700 Bioanalyzer,and there were 75 differential expressed genes with the CML disease of the two samples. Results showed that ABI 1700 Bioanalyzer can be instrumental for the study of gene differential expression. |
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| Keywords: | ABI 1700 Bioanalyzer differential expression gene CML |
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