18B1: An Axon-Specific Antigen Associated with Many Proteins |
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Authors: | Richard Hawkes Evelyn Niday rew Matus |
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Affiliation: | Friedrich Miescher-Institut, Basel, Switzerland |
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Abstract: | We describe an antigen, 18B1, defined by a monoclonal antibody. Immunoperoxidase staining of brain sections shows that 18B1 is selectively associated with neurofilament-rich axons. Antibody staining of sodium dodecyl sulphate-gel blots, on the other hand, shows that 18B1 is associated with a large number of proteins, none of which are structural components of brain neurofilaments. The antigen is sensitive to a variety of proteases but is not degraded by various glycosidases, suggesting that 18B1 is probably an amino acid sequence. Its association with neurofilament-rich axons together with its absence from neurofilaments themselves suggests that it may be involved in mediating interactions between neurofilaments and the proteins that bear it. |
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Keywords: | Monoclonal antibody Axonal transport Nervous system Immunohistochemistry Differentiation |
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