Elimination of channel-forming activity by insertional inactivation of the p66 gene in Borrelia burgdorferi |
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Authors: | Pinne Marija Thein Marcus Denker Katrin Benz Roland Coburn Jenifer Bergström Sven |
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Institution: | Department of Molecular Biology, Ume? University, Sweden. |
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Abstract: | P66 is a chromosomally encoded 66-kDa integral outer membrane protein of the Lyme disease agent Borrelia burgdorferi exhibiting channel-forming activity. Herein, we inactivated and subsequently complemented the p66 gene in the B31-A (WT) strain. The P66 protein was also inactivated in two other channel-forming protein mutant strains, P13-18 (Deltap13) and Deltabba01, and then compared with the channel-forming activities of wild-type and various p66 mutant strains. We further investigated the ion-selectivity of native, purified P66. In conclusion, we show that the porin activity of P66 is eliminated by insertional inactivation and that this activity can be rescued by gene complementation. |
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Keywords: | channel-forming activity P66 porin insertional inactivation Borrelia burgdorferi |
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