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Biotransformation of oleic acid into 10-hydroxy-8E-octadecenoic acid by Pseudomonas sp. 42A2 |
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| Authors: | J Bastida C de Andrés J Culleré M Busquets A Manresa |
| Institution: | (1) Departamento de Ingeniería Química-Murcia, Universidad de Murcia, Campus del Espinardo, Murcia, Spain;(2) Laboratori de Microbiología, Facultat de Farmàcia, Universitat de Barcelona, 08028 Barcelona, Spain;(3) Departamento de Bioquímica i Biologia Celular, Facultat de Quimica, Universitat de Barcelona, Spain |
| Abstract: | Pseudomonas sp. 42A2 when incubated for 36 h with oleic acid (20 g l–1) in a stirred bioreactor, accumulated 10-hydroxy-8E-octadecenoic acid. Production in a 2 l bioreactor with 1.4 l of working volume, was increased from 0.65 g l–1 to 7.4 g l–1 with K L a values ranging between 15 and 200 h–1. A linear relationship was found between volumetric productivity and oxygen transfer rates and an exponential relation between the specific rate of product formation and specific growth rate. |
| Keywords: | biotransformation oleic acid Pseudomonas sp 42A2 10-hydroxy-8E-octadecenoic acid |
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