Bitter melon seed oil increases mitochondrial content in gastrocnemius muscle and improves running endurance in sedentary C57BL/6J mice |
| |
| Institution: | 1. Department of Nutrition, China Medical University, Taichung, Taiwan;2. Department of Exercise Health Science, National Taiwan University of Sport, Taichung, Taiwan;3. Department of Public Health, College of Public Health, China Medical University, Taichung, Taiwan;4. Department of Healthcare Administration, College of Medical and Health Science, Asia University, Taichung, Taiwan;5. Aquavan Technology Co., Ltd., Taipei City, Taiwan;1. Department of Chemistry, College of Natural Sciences, Seoul National University, Seoul 08826, Republic of Korea;2. Research Institute of Pharmaceutical Sciences, College of Pharmacy, Seoul National University, Seoul 08826, Republic of Korea;3. Biomolecular Function Research Branch, Division of Precision Medicine and Cancer Informatics, Research Institute, National Cancer Center, Gyeonggi 10408, Republic of Korea;1. NIH Chemical Genomics Center, National Center for Advancing Translational Sciences, National Institutes of Health, Bethesda, MD, USA;2. National Toxicology Program, National Institutes of Environmental Health Sciences, National Institutes of Health, Research Triangle Park, NC, USA;3. PamGene International B.V., Wolvenhoek 10, 5211 HH ''s-Hertogenbosch, The Netherlands;4. Integrated Laboratory System, Inc., Morrisville, NC, USA |
| |
| Abstract: | The α-eleostearic acid (α-ESA) in bitter melon seed oil (BMSO) is efficiently converted by the body into rumenic acid. The objective of this study was to investigate effects of BMSO on skeletal muscle fiber-type switch and endurance capacity in mice, with or without exercise training. In a 3×2 factorial design, C57BL/6J mice were fed a 30% high-fat diet composed of soybean oil, butter or a 1:1 mixture of BMSO and soybean oil, i.e., SB, BT and BM diets, respectively, and were allocated to be sedentary or undergo exercise (Ex). The Ex groups received a 15-min training regimen on a motorized treadmill 5 times a week. After 3-week intervention, endurance capacity was evaluated (total running time and distance until exhaustion). Mice fed a BM diet had significantly less body fat, with increased muscle percentage and improved endurance capacity. Combining sedentary and Ex groups, mice fed a BM diet ran 33% longer and 50% further than those fed SB, or 25% longer and 36% further than those fed BT (P<.01). The BM-diet-increased gastrocnemius cytochrome c protein and mitochondrial DNA content was more prominent in sedentary than in trained mice. Histochemical staining shows sedentary BM-fed mice had a higher succinate dehydrogenase activity among groups. Based on a reporter assay, rumenic acid, rather than α-ESA itself, activated PPARδ ligand binding domain. We concluded that BMSO improved endurance capacity via stimulation of mitochondrial biogenesis and function, potentially influencing muscle metabolism and fiber-type composition in sedentary mice. |
| |
| Keywords: | |
| 本文献已被 ScienceDirect 等数据库收录！ |
|
