Protein-restriction diet during the suckling phase programs rat metabolism against obesity and insulin resistance exacerbation induced by a high-fat diet in adulthood |
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| Affiliation: | 1. Laboratory of Secretion Cell Biology, Department of Biotechnology, Genetics and Cell Biology, State University of Maringa, Maringá/PR, Brazil;2. Institute of Health Sciences, Federal University of Mato Grosso, Sinop, MT, Brazil;3. Carlos Chagas Filho Biophysics Institute, Federal University of Rio de Janeiro, Rio de Janeiro/RJ, Brazil;1. Laboratory of Endocrine Physiology and Metabolism, Federal University of Goiás, Brazil;2. Neuroscience and Cardiovascular Physiology Research Center, Federal University of Goiás, Brazil;3. Integrative Laboratory of Cardiovascular and Neurological Pathophysiology, Federal University of Goiás, Brazil;4. Laboratory of Biology of Cellular Secretion, State University of Maringá, Brazil;5. Laboratory of Systems Neurobiology, Federal University of Goiás, Brazil;1. Depto. de Fisiología de la Nutrición, Instituto Nacional de Ciencias Médicas y Nutrición “Salvador Zubirán”, Ciudad de México, Mexico;2. Facultad de Química, Universidad Nacional Autónoma de México, Ciudad de México, Mexico;1. Department of Cardiology and Clinical Laboratory, Tokyo Metropolitan Komagome Hospital, Japan;2. Division of Cardiology, Department of Internal Medicine, Nippon Medical School Chiba Hokusoh Hospital, Japan;3. Department of Internal Medicine, Nippon Medical School, Japan |
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| Abstract: | Protein restriction during the suckling phase can malprogram rat offspring to a lean phenotype associated with metabolic dysfunctions later in life. We tested whether protein-caloric restriction during lactation can exacerbate the effect of a high-fat (HF) diet at adulthood. To test this hypothesis, we fed lactating Wistar dams with a low-protein (LP; 4% protein) diet during the first 2 weeks of lactation or a normal-protein (NP; 23% protein) diet throughout lactation. Rat offspring from NP and LP mothers received a normal-protein diet until 60 days old. At this time, a batch of animals from both groups was fed an HF (35% fat) diet, while another received an NF (7% fat) diet. Maternal protein-caloric restriction provoked lower body weight and fat pad stores, hypoinsulinemia, glucose intolerance, higher insulin sensitivity, reduced insulin secretion and altered autonomic nervous system (ANS) function in adult rat offspring. At 90 days old, NP rats fed an HF diet in adulthood displayed obesity, impaired glucose homeostasis and altered insulin secretion and ANS activity. Interestingly, the LP/HF group also presented fat pad and body weight gain, altered glucose homeostasis, hyperleptinemia and impaired insulin secretion but at a smaller magnitude than the NP-HF group. In addition, LP/HF rats displayed elevated insulin sensitivity. We concluded that protein-caloric restriction during the first 14 days of life programs the rat metabolism against obesity and insulin resistance exacerbation induced by an obesogenic HF diet. |
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