A diet defined by its content of bovine milk exosomes and their RNA cargos has moderate effects on gene expression,amino acid profiles and grip strength in skeletal muscle in C57BL/6 mice |
| |
| Affiliation: | 2. Department of Computer Science & Engineering, University of Nebraska-Lincoln, 256 Avery Hall, 1144 T Street, Lincoln, NE 68588-0115, USA;3. Department of Biochemistry, University of Nebraska-Lincoln, N200 Beadle Center, 1901 Vine Street, Lincoln, NE 68588-0664, USA;1. Department of Pediatric Endocrinology and Genetic Metabolism, Shanghai Institute for Pediatric Research, Xinhua Hospital, School of Medicine, Shanghai Jiao Tong University, Shanghai, China;2. Department of Pediatric Surgery, Children''s Hospital of Fudan University, Shanghai, China;3. National Children''s Medical Center, Shanghai, China;4. Translational Medicine Program, The Hospital for Sick Children, Toronto, ON, Canada;5. Division of General and Thoracic Surgery, The Hospital for Sick Children, Toronto, ON, Canada;6. Clinical Research Unit, Children''s Hospital of Fudan University, Shanghai, China;7. Department of Surgery, University of Toronto, Toronto, ON, Canada;1. CHU de Québec Research Center/CHUL Pavilion, 2705 Blvd Laurier, Quebec City, QC, G1V 4G2 and Department of Microbiology, Infectious Diseases and Immunology, Faculty of Medicine, Université Laval, Quebec City, QC G1V 0A6, Canada;2. Proteomics Platform, Genomics Center, CHU de Québec Research Center/CHUL Pavilion, 2705 Blvd Laurier, Quebec City, QC G1V 4G2, Canada |
| |
| Abstract: | Exosomes are nanoparticles that transfer cargos from donor cells to recipient cells where they elicit changes in gene expression and metabolism. Evidence suggests that exosomes and their cargos are also absorbed from dietary sources such as bovine milk, and bovine exosomes promote the growth of myofibers in murine C2C12 myotube cell cultures. The aim of the current study was to determine whether the dietary intake of bovine milk exosomes alters strength, gene expression and amino acid profiles in murine skeletal muscles. Male and female C57BL/6 mice, age three weeks, were fed an AIN93G-based, exosome and RNA-depleted (ERD) diet for six weeks; controls were fed an exosome and RNA-sufficient (ERS) diet. Variables of feeding behavior, metabolism, grip strength, liver and kidney function, amino acid profiles, and gene expression patterns were analyzed by using metabolic cages, grip strength analyzers, clinical chemistry analyzers, targeted LC/MS–MS, and RNA sequencing analysis. The diets had no effect on food and water intake, respiratory exchange rate, physical activity, grip strength, markers of liver and kidney dysfunction, and amino acid profiles in muscle. Only twelve and nine mRNAs were differentially expressed in skeletal muscle from female and male mice, respectively, fed ERD and ERS diets. The modest effect of the ERD diet on gene expression and levels of free amino acids in skeletal muscle is consistent with observations that bovine milk exosomes and their cargos accumulate in tissues other than skeletal muscle. |
| |
| Keywords: | |
| 本文献已被 ScienceDirect 等数据库收录！ |
|
