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| 凝集素法测定骨性碱性磷酸酶及临床应用 | |
| 引用本文： | 彭志英,东云华,韦晓明,郑虎.凝集素法测定骨性碱性磷酸酶及临床应用[J].生物化学与生物物理进展,2000,27(4):448-451. |
| 作者姓名： | 彭志英 东云华 韦晓明 郑虎 |
| 作者单位： | 1. 华西医科大学附属第一医院检验科,成都,610041 2. 华西医科大学分析测试中心,成都,610041 3. 华西医科大学医检专业94级,成都,610041 4. 华西医科大学药学院,成都,610041 |
| 摘 要： | 选用凝集素分离法测定骨性碱性磷酸酶（bone alkaline phosphatase，B-ALP）.分别对健康男女儿童123例和成人及老年66例，进行B-ALP和总ALP活性的测定.结果显示，在儿童骨生长阶段B-ALP水平随年龄增长而增高，到14～16岁时开始逐渐下降至成人水平，男女间增高和下降时间有所不同.同时对临床骨折病人60例测定以上项目，表现为B-ALP活性在骨折后一段时期内升高，提示骨形成活跃.粉碎性骨折两周后B-ALP活性有显著性差异，t=2.92, P＜0.01，而裂缝性骨折三周后出现显著性，t=5.14, P＜0.01.骨折病人自身比较，一周后出现显著性差异, t=3.51, P＜0.05.凝集素法操作简便、重复性好，高低浓度血清的批内、批间变异系数分别为6.12%、8.5%和6.4%、9.5%.是临床观察骨代谢情况的一项有用指标. |
| 关 键 词： | 骨性碱性磷酸酶，凝集素，骨折 |
| 收稿时间： | 1999/11/9 0:00:00 |
| 修稿时间： | : |
Determination of Alkaline Phosphatase Activity by the Agglutinin Precipitation Assay and Its Clinical Application |
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| PENG Zhi-Ying,DONG Yun-Hu,WEI Xiao-Ming and ZHENG Yong.Determination of Alkaline Phosphatase Activity by the Agglutinin Precipitation Assay and Its Clinical Application[J].Progress In Biochemistry and Biophysics,2000,27(4):448-451. | |
| Authors: | PENG Zhi-Ying DONG Yun-Hu WEI Xiao-Ming and ZHENG Yong |
| Institution: | First Hospital Laboratory Medicine of West China University of Medical Science, Chengdu 610041, China;Analyze and Determine Center of West China University of Medical Science, Chengdu 610041, China;Department of Laboratory Medicine, West China University of Medical Science, Chengdu 610041, China;College of Pharmacology, West China University of Medical Science, Chengdu 610041, China |
| Abstract: | The agglutinin precipitation assay was used to determine the bone alkaline phosphatase. The advantage of this assay is simple manipulation, and good reproducibility. Coefficient variation within and between runs were 6.12%, 8.5% and 6.4%, 9.5% respectively. It is concluded that serum B-ALP levels is an useful parameter for clinical observation of the bone metabolism. |
| Keywords: | bone alkaline phophatase agglutinin bone fracture |
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