Peptidergic motoneurons in the buccal ganglia of Aplysia californica Immunocytochemical,morphological, and physiological characterizations |
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Authors: | Paul J Church Kevin P Cohen Marsha L Scott Mark D Kirk |
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Institution: | (1) University of Missouri-Columbia, Division of Biological Sciences, Lefevre Hall, 65211 Columbia, MO, USA;(2) Present address: Committee on Neurobiology, University of Chicago, 947 E. 58th St., 60637 Chicago, IL, USA |
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Abstract: | Summary We used physiological recordings, intracellular dye injections and immunocytochemistry to further identify and characterize neurons in the buccal ganglia of Aplysia calif ornica expressing Small Cardioactive Peptide-like immunoreactivity (SCP-LI). Neurons were identified based upon soma size and position, input from premotor cells B4 and B5, axonal projections, muscle innervation patterns, and neuromuscular synaptic properties. SCP-LI was observed in several large ventral neurons including B6, B7, B9, B10, and B11, groups of s1 and s2 cluster cells, at least one cell located at a branch point of buccal nerve n2, and the previously characterized neurons B1, B2 and B15.B6, B7, B9, B10 and B11 are motoneurons to intrinsic muscles of the buccal mass, each displaying a unique innervation pattern and neuromuscular plasticity. Combined, these motoneurons innervate all major intrinsic buccal muscles (I1/I3, I2, I4, I5, I6). Correspondingly, SCP-LI processes were observed on all of these muscles. Innervation of multiple nonhomologous buccal muscles by individual motoneurons having extremely plastic neuromuscular synapses, represents a unique form of neuromuscular organization which is prevalent in this system. Our results show numerous SCPergic buccal motoneurons with widespread ganglionic processes and buccal muscle innervation, and support extensive use of SCPs in the control of feeding musculature.Abbreviations
SCP-LI
small cardioactive peptide-like immunoreactivity
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PSC
postsynaptic current
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EPSP
excitatory postsynaptic potential
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IPSP
inhibitory postsynaptic potential
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FI
facilitation index
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TMR
time to maximal response |
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Keywords: | Aplysia Motoneurons Immunocytochemistry Small cardioactive peptides Facilitation Depression Buccal Feeding |
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