The impact of Zika virus exposure on the placental proteomic profile |
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| Affiliation: | 1. GlycoProteomics Laboratory, Department of Parasitology, Department of Parasitology, Institute of Biomedical Sciences, University of Sao Paulo, Sao Paulo, Brazil;2. Laboratory for Studies in Maternal-Fetal Interactions and Placenta, Department of Cell and Developmental Biology, Institute of Biomedical Sciences, University of Sao Paulo, SP, Brazil;3. Department of Microbiology, Institute of Biomedical Sciences, University of Sao Paulo, Sao Paulo, SP, Brazil;4. Huntington Medicina Reprodutiva, São Paulo, SP, Brazil;5. Faceres School of Medicine, São José do Rio Preto, SP, Brazil;6. Laboratory of Experimental Immunoparasitology, Department of Parasitology, Institute of Biomedical Sciences, University of Sao Paulo, SP, Brazil;7. Biochemistry and Molecular Biology Department, University of Southern Denmark, Odense, Denmark |
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| Abstract: | Zika virus (ZIKV) infection has caused severe unexpected clinical outcomes in neonates and adults during the recent outbreak in Latin America, particularly in Brazil. Congenital malformations associated with ZIKV have been frequently reported; nevertheless, the mechanism of vertical transmission and the involvement of placental cells remains unclear. In this study, we applied quantitative proteomics analysis in a floating explant model of chorionic villi of human placental tissues incubated with ZIKV and with ZIKV pre-adsorbed with anti-ZIKV envelope protein. Proteomic data are available via ProteomeXchange with identifier PXD025764. Altered levels of proteins were involved in cell proliferation, apoptosis, inflammatory processes, and the integrin-cytoskeleton complex. Antibody-opsonized ZIKV particles differentially modulated the pattern of protein expression in placental cells; this phenomenon may play a pivotal role in determining the course of infection and the role of mixed infections. The expression of specific proteins was also evaluated by immunoperoxidase assays. These data fill gaps in our understanding of early events after ZIKV placental exposure and help identify infection control targets. |
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