Studies on the macroconidia of Microsporum canis

The characteristics of an in vitro polyuridylic acid dependent amino acid incorporating system prepared from germinating macroconidia of Microsporum canis are described. The incorporation of ¹⁴C-phenylalanine into polyphenylalanine is dependent on S-30 extract, adenosine triphosphate, magnesium ions and polyuridylic acid. Incorporation is slightly enhanced by yeast transfer ribonucleic acid and pyruvate kinase. The system is highly sensitive to ribonuclease, puromycin and miconazole (an antifungal agent), moderately sensitive to sodium fluoride and much less sensitive to phenethylalcohol, cycloheximide, chloramphenicol and deoxyribonuclease. Cell-free extract from ungerminated conidia has less capacity to synthesize the protein and during germination a marked increase in the protein synthetic activity is observed. The results from experiments wherein ribosomes and S-100 fraction from germinated and ungerminated spores are interchanged, revealed that the defect in the extract from the ungerminated spore is in the ribosomes.Abbreviations Poly(U) polyuridylic acid - tRNA transfer ribonucleic acid - ATP adenosine triphosphate - GTP guanosine triphosphate - BSA bovine serum albumin - RNase ribonuclease - DNase deoxyribonuclease - POPOP 1,4-bis-2(5-phenyl oxazolyl)benzene - PPO 2,5-diphenyl oxazole - TCA trichloracetic acid