Radioimmunoassay of 17-hydroxyprogesterone

A method is described for the determination of 17-hydroxyprogesterone in peripheral venous plasma (0.1–1.0 ml) from men and women using an antiserum to 17-hydroxyprogesterone-3-carboxymethyl oxime bovine serum albumin (BSA).

The coefficients of variation on replicate analyses ranged from 7–16%. The louest level of 17-hydroxy-progesterone uhich may be determined is 5 ng/100 ml plasma. The concentration (mean ± S.D.; ng/100 ml plasma) in a group of healthy men (aged 20–40 yrs) uas 123 ± 65. From women during days 1–10 of the menstrual cycle the value uas 40 ± 15, during days 18–32 of the cycle 134 ± 57 and during pregnancy (12th week to term) 622 ± 262. Progesterone was determined in the same samples using an antiserum to 11-hydroxyprogesterone-11-hemisuccinate-BSA.