Uptake of glycine froml-alanylglycine into renal brush border vesicles

Summary Isolated renal brush border microvilli vesicles were employed to study the uptake of radiolabel froml-Ala · ³H]Gly andd-Ala · ³H]Gly as well as to determine the presence of dipeptidase activity. Microvilli vesicles were prepared from porcine kidney cortex by differential centrifugation through hypotonic Tris buffer containing Mg²⁺. The microvilli vesicles transiently accumulated radiolabel froml-Ala · ³H]Gly to higher levels than were initially present in the incubation medium (overshoot phenomenon). This accumulation was dependent on the presence of an inward-directed (extravesicular > intravesicular) Na⁺ gradient and was osmotically sensitive and linear with respect to microvilli protein concentration. Analysis of intravesicular contents revealed that all³H uptake froml-Ala · ³H]Gly appeared as free glycine. Hydrolysis studies demonstrated the rate ofl-ala · ³H]Gly hydrolysis to free alanine and ³H] glycine by the microvilli to be greatly in excess of their rate of radiolabel uptake from this dipeptide. In addition, the uptake profiles and kinetic constants for vesicular uptake of radiolabel froml-Ala · ³H]Gly and free glycine were demonstrated to be identical when measured by double-labeling techniques in the same experiments. These results indicate thatl-Ala · ³H]Gly is hydrolyzed at the external surface of the microvilli with the ³H]glycine released being transported into the vesicles by a Na⁺ gradient-dependent system identical to that employed for free glycine.Microvilli vesicle uptake of radiolabel fromd-Ala · ³H]Gly exhibited no Na⁺ dependent overshoot effect.d-Ala · ³H]Gly was completely resistant to microvilli-catalyzed hydrolysis.Analysis of the microvilli for renal dipeptidase, an enzyme with hydrolytic activity against a wide range ofl-dipeptides, revealed this enzyme to be enriched in the microvilli vesicles to a degree equivalent to that observed for marker enzymes for renal microvilli.Renal dipeptidase catalyzed hydrolysis ofl-Ala · Gly but notd-Ala · Gly, as was the case with microvilli-catalyzed hydrolysis of these dipeptides.With its location in the renal brush border microvilli and its hydrolytic action againstl-dipeptides, renal dipeptidase may act at the luminal surface of the proximal tubule cell to hydrolyzel-dipeptides present in the glomerular filtrate, with the resultant free amino acids transported across the brush border microvilli by Na⁺ gradient-dependent processes.