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Structural elucidation of a neutral fucogalactan from the mycelium of Coprinus comatus |
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| Authors: | Fan JunMin Zhang JingSong Tang QingJiu Liu YanFang Zhang AnQiang Pan YingJie |
| Institution: | College of Life Sciences, Nanjing Agricultural University, 210095 Nanjing, China. |
| Abstract: | A water-soluble fucogalactan (CMP3), with a molecular mass of 1.03 x 10(4) Da as determined by high-performance size-exclusion chromatography (HPSEC), was obtained from the crude intracellular polysaccharide of Coprinus comatus mycelium. Its chemical structure was characterized by sugar and methylation analysis along with 1H and 13C NMR spectroscopy, including NOESY and HMBC experiments for linkage and sequence analysis. The polysaccharide is composed of a pentasaccharide repeating unit with the following structure: structure:see text]. |
| Keywords: | NMR nuclear magnetic resonance COSY correlation spectroscopy TOCSY total correlation spectroscopy HMQC heteronuclear multiple-quantum coherence spectroscopy NOESY nuclear Overhauser effect spectroscopy HPLC high-performance liquid chromatography HPAEC-PAD high-performance anion exchange-pulse amperometric detection chromatography |
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