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Development of 120 microsatellite markers for Primula sieboldii E. Morren for linkage mapping |
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| Authors: | Saneyoshi Ueno Yasuko Yoshida Yuriko Taguchi Masanori Honjo Naoko Kitamoto Izumi Washitani Ryo Ohsawa Yoshihiko Tsumura |
| Affiliation: | 1. Tree Genetics Laboratory, Department of Forest Genetics, Forestry and Forest Products Research Institute, 1 Matsunosato, Tsukuba, Ibaraki, 305-8687, Japan 2. Graduate School of Life and Environmental Sciences, University of Tsukuba, 1-1-1 Tennoudai, Tsukuba, Ibaraki, 305-8572, Japan 3. National Agricultural Research Center for Tohoku Region, 4 Akahira, Shimo-kuriyagawa, Morioka, Iwate, 020-0198, Japan 4. Graduate School of Agricultural and Life Science, The University of Tokyo, 1-1-1 Yayoi, Bunkyo-ku, Tokyo, 113-8657, Japan |
| Abstract: | Primula sieblodii E. Morren is a perennial herb, classified as ‘Near Threatened’ in the Japanese Red List. Using the magnetic enrichment method, we have developed 120 microsatellite markers and screened polymorphisms within the species as a whole as well as within a single population. At the species level, the average number of alleles per locus, and the average observed and expected heterozygosities were 7.06, 0.48, and 0.74, respectively; at the level of the single population these values were 5.53, 0.52, and 0.64, respectively. The microsatellite markers presented here will be valuable research tools in forthcoming projects, such as QTL mapping as well as for association studies of P. sieboldii. |
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