Crystallization of Ribulose, 1,5-Bisphosphate Carboxylase of High Specific Activity from Anther-Derived Haploid Plants of Nicotiana tabacum

Haploid plants were generated from anther-culture of Nicotianatabacum cv. Burley Ky 14. Plants that developed from pollenwithout an intervening callus stage were grown to maturity.Ploidy of the anther-derived plants were verified by chromosomecounts in root tip squashes. Crystalline ribulose 1,5-bisphosphatecarboxylase was purified from leaves of mature haploid plantsgrown in a growth chamber. Crystals appeared to be similar tothose from the diploid parental line. The specific activityof the enzyme was higher than those reported earlier in theliterature using similar crystallization procedures in Nicotianatabacum Key words: Nicotiana, haploid, ribulose 1, 5-bisphosphate carboxylase