Increased adherence of human granulocytes to herpes simplex virus type 1 infected endothelial cells |
| |
Authors: | Barbara A Zajac Karen O'Neill Harvey M Friedman Rob Roy Mac Gregor |
| |
Institution: | (1) Infectious Diseases Section, Department of Medicine, University of Pennsylvania School of Medicine, 536 Johnson/G2, 19104 Philadelphia, PA;(2) The Joseph Stokes Jr. Research Institute, Childrens' Hospital of Philadelphia, Pennsylvania |
| |
Abstract: | Summary We studied the interaction of human polymorphonuclear leukocytes (PMNs) with umbilical vein endothelial cells infected with
herpes simplex virus (HSV) type 1. PMNs labeled with51Cr were added to endothelial monolayers at varying times after infection and their adherence assessed 1 h later. Granulocyte
adherence (GA) to uninfected cells averaged 26.5±1.9%. Increased adherence began 6 h postinfection and rose to a maximum at
20 to 24 h. HSV-1 glycoproteins seemed to mediate the increase in GA: tunicamycin treatment of infected monolayers for 18
h abolished the increased GA as did incubation of infected cells with F(ab')2 fragments prepared from human antiserum containing HSV-1 antibody.
Supported by grants R01-AA-06029 and T32-AA07233 from the National Institute of Alcohol Abuse and Alcoholism, and R01-HL-28220
from the National Heart, Lung, and Blood Institute. |
| |
Keywords: | endothelium granulocyte inflammation virus infection |
本文献已被 SpringerLink 等数据库收录! |
|