Irradiated tumor cells adenovirally engineered to secrete granulocyte/macrophage-colony-stimulating factor establish antitumor immunity and eliminate pre-existing tumors in syngeneic mice |
| |
Authors: | Eishi Nagai Takahiro Ogawa Tammy Kielian Akashi Ikubo Tsuneo Suzuki |
| |
Institution: | (1) Department of Microbiology, Molecular Genetics and Immunology, University of Kansas Medical Center, 3901 Rainbow Blvd., Kansas City, KS 66160-7420, USA e-mail: tsuzuki@kumc.edu Tel.:+1-913-588-6724 or 7099; Fax:+1-913-588-7295, US |
| |
Abstract: | The specific aim of this study was to examine the prophylactic as well as the therapeutic efficacies of irradiated mouse
CT26 colon cancer cells, infected with recombinant adenoviruses harboring cDNAs specific for granulocyte macrophage-colony-stimulating
factor (GM-CSF), interferon (IFN-γ) and monocyte chemotactic protein1 (MCP-1). Results showed that tumor cells secrete the
respective cytokines for several days after infection and subsequent irradiation. Vaccination with irradiated GM-CSF-secreting
CT26 cells protected 90% of syngeneic mice challenged with live parental cells. On the other hand, vaccination with irradiated
IFNγ or MCP-1-secreting CT26 cells totally failed to protect mice from tumor development after challenge with parental cells.
None of the tumor-free mice initially vaccinated with irradiated GM-CSF-producing CT26 cells developed tumor upon repeated
challenge with parental cells during the entire observation period. The establishment of specific and long-lasting antitumor
immunity following vaccination with GM-CSF-producing tumor cells requires the simultaneous presence of GM-CSF and tumor antigen
at the vaccine site. Depletion of CD8+ cells, but not CD4+ cells, blocked the vaccine efficacy of GM-CSF-producing tumor cells. Subcutaneous injection of irradiated GM-CSF-producing
CT26 cells also effectively prevented the growth of a small load of parental tumor that was implanted 3 days earlier or the
development of metastatic foci in the lung from intravenously injected parental cells either 7 days before or 3 days after
vaccination. Our data thus show that, in these experimental tumor models, subcutaneous injection of irradiated tumor cells
adenovirally, transduced with the GM-CSF gene leads not only to prevention of growth of subsequently implanted tumor but also
to elimination of pre-existing and metastatic tumors. |
| |
Keywords: | GM-CSF gene Adenovirus Cancer Vaccine Therapy |
本文献已被 SpringerLink 等数据库收录! |
|