Sporulation of Clostridium botulinum Type E in Different Culture Media

Twenty-seven strains of Clostridium botulinum type E were inoculated into six culture media and examined for production of spores. Thirty-six similar media were then inoculated with each of five strains. Strains varied greatly in their ability to form spores in a given medium. Spore formation varied markedly with casein digests and peptones of different manufacturers. Some differences were broadly indicated between strains from the Pacific and from Scandinavia.