Comparative ELISA reagents for detection of hepatitis B surface antigen (HBsAg) |
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| Authors: | I F Camargo A M Gaspar C F Yoshida |
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| Affiliation: | Bio-Manguinhos-FIOCRUZ, Rio de Janeiro, Brasil. |
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| Abstract: | Conjugates of goat anti-HBs IgG and horseradish peroxidase (HRP) prepared by two different methods, one using NaIO4 and the other SPDP, were compared. Anti-HBs antibodies obtained from goat, rabbit and guinea-pig were tested as capture serum. The ELISA showed a sensitivity similar to RIA and a level of antigen captation ranging from 4.37 to 8.75 nanograms/ml was obtained when rabbit or guinea-pig captures were used combined with both NaIO4 or SPDP conjugates. |
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