Probing endogenous collagen by laser‐induced autofluorescence in burn wound biopsies: A pilot study |
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Authors: | Vijendra Prabhu Anusha Acharya Bola S. Satish Rao Bharath Rathnakar Pramod Kumar Vasudeva Guddattu Krishna K. Mahato |
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Affiliation: | 1. Department of Biophysics, School of Life Sciences, Manipal Academy of Higher Education, Manipal, India;2. Department of Biotechnology, School of Life Sciences, Manipal Academy of Higher Education, Manipal, India;3. Department of Radiation Biology and Toxicology, School of Life Sciences, Manipal Academy of Higher Education, Manipal, India;4. Department of Plastic Surgery and Burns, Kasturba Medical College, Manipal Academy of Higher Education, Manipal, India;5. Department of Statistics, Prasanna School of Public Health, Manipal Academy of Higher Education, Manipal, India |
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Abstract: | The focus of the current study was to interrogate the predictive potential of laser‐induced autofluorescence (LIAF) by objectively assessing collagen synthesis in burn wound granulation tissues ex vivo. Prior grafting, granulation tissues (20 samples) following burn injury were collected from 17 subjects of age range 18 to 60 years with patient/donor consent and the corresponding autofluorescence spectra were recorded at 325 nm He‐Cd laser (≈2 mW) excitations. The resulting endogenous collagen intensity from the above tissue samples was computed by normalizing the nicotinamide adenine dinucleotide levels. In addition, the hydroxyproline content was also estimated biochemically from the same granulation tissues. A comparative assessment of both LIAF and biochemical estimations for endogenous collagen by hydroxyproline resulted in strong positive correlation among them. The above relevant observations suggest that LIAF is equally informative as that of biochemical estimations, in evaluating endogenous collagen content in wound granulation tissues. Thus, it can be concluded that LIAF has the predictive potential, as a noninvasive objective tool to measure the endogenous collagen levels in wound biopsy tissues and provide complementary data conducive for making clinical decisions. |
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Keywords: | burn wounds endogenous collagen laser‐induced autofluorescence objective assessment predictive potential |
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