Inside Cover: Internalization kinetics and cytoplasmic localization of functionalized diatomite nanoparticles in cancer cells by Raman imaging (J. Biophotonics 4/2018)
1. Institute of Protein Biochemistry, National Research Council of Italy, Naples, Italy;2. Department of Molecular Medicine and Medical Biotechnology, University Federico II of Naples, Naples, Italy;3. Institute for Microelectronics and Microsystems, Udr Naples, National Research Council of Italy, Naples, Italy
Abstract:
The internalization kinetics and intracellular spatial distribution of functionalized diatomite nanoparticles in human lung epidermoid carcinoma cell line have been investigated by confocal fluorescence and Raman microscopy. In this context, Raman imaging due to its non‐destructive, chemically selective and label‐free working principle provides evidence that the nanovectors are internalized and co‐localize with lipid environments, suggesting an endocytic internalisation route. Nanoparticle uptakes and intracellular persistence are observed up to 72 hours, without damage to cell viability or morphology. Further details can be found in the article by Stefano Managò et al. ( e201700207 )
