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Inside Front Cover: Combining hyperspectral imaging and chemometrics to assess and interpret the effects of environmental stressors on zebrafish eye images at tissue level (J. Biophotonics 3/2018) |
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| Authors: | Víctor Olmos Mònica Marro Pablo Loza‐Alvarez Demetrio Raldúa Eva Prats Francesc Padrós Benjamin Piña Romà Tauler Anna de Juan |
| Affiliation: | 1. Department of Chemical Engineering and Analytical Chemistry, University of Barcelona, Barcelona, Spain;2. Institut de Ciencies Fotòniques (ICFO), The Barcelona Institute of Science and Technology, Castelldefels, Spain;3. Department of Environmental Chemistry, Institute of Environmental Assessment and Water Diagnostic (IDAEA‐CSIC), Barcelona, Spain;4. Research and Development Centre (CID‐CSIC), Barcelona, Spain;5. Pathological Diagnostic Service in Fish, Universitat Autònoma de Barcelona, Bellaterra, Spain |
| Abstract: | Raman images were used to study the effect of the contaminant chlorpyriphos‐oxon on zebrafish eye samples. Multivariate Curve Resolution‐Alternating Least Squares (MCR‐ALS) was used to obtain the distribution maps and spectral signatures of biological components present in the images analyzed. The use of MCRALS spectral signatures as starting information for Partial Least Squares‐Discriminant Analysis allowed statistical assessment of the effect of the contaminant at a specific tissue level. Further details can be found in the article by Víctor Olmos et al. ( e201700089 ).  |
| Keywords: | MCR‐ALS PLS‐DA Raman HSI resampling zebrafish |