Correcting the limited view in optical‐resolution photoacoustic microscopy |
| |
| Authors: | Wei Liu Yuan Zhou Mengran Wang Lei Li Emelina Vienneau Ruimin Chen Jianwen Luo Chris Xu Qifa Zhou Lihong V. Wang Junjie Yao |
| |
| Affiliation: | 1. Department of Biomedical Engineering, Duke University, Durham, North Carolina;2. Department of Biomedical Engineering, School of Medicine, Tsinghua University, Beijing, China;3. School of Applied and Engineering Physics, Cornell University, Ithaca, New York;4. Caltech Optical Imaging Laboratory, Andrew and Peggy Cherng Department of Medical Engineering, Department of Electrical Engineering, California Institute of Technology, Pasadena, California;5. Department of Biomedical Engineering, University of Southern California, Los Angeles, California |
| |
| Abstract: | Optical‐resolution photoacoustic microscopy (OR‐PAM) has proven useful for anatomical and functional imaging with high spatial resolutions. However, the coherent signal generation and the desired reflection‐mode detection in OR‐PAM can result in a limited detectability of features aligned with the acoustic axis (ie, vertical structures). Here, we investigated the limited‐view phenomenon in OR‐PAM by simulating the generation and propagation of the acoustic pressure waves and determined the key optical parameters affecting the visibility of vertical structures. Proof‐of‐concept numerical experiments were performed with different illumination angles, optical foci and numerical apertures (NA) of the objective lens. The results collectively show that an NA of 0.3 can readily improve the visibility of vertical structures in a typical reflection‐mode OR‐PAM system. This conclusion was confirmed by numerical simulations on the cortical blood vessels in a mouse brain and by experiments in a suture‐cross phantom and in a mouse brain in vivo.  |
| |
| Keywords: | coherent signal K‐wave limited view mouse brain photoacoustic imaging |
|
|
