Enrichment, isolation and characterization of pentachlorophenol degrading bacterium Acinetobacter sp. ISTPCP-3 from effluent discharge site |
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Authors: | Ashwani Sharma Indu Shekhar Thakur Prem Dureja |
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Institution: | (1) School of Environmental Sciences, Jawaharlal Nehru University, New Mehrauli road, New Delhi, 110067, India;(2) Division of Agricultural Chemicals, Indian Agricultural Research Institute, New Delhi, 110012, India |
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Abstract: | Three pentachlorophenol (PCP) degrading bacterial strains were isolated from sediment core of pulp and paper mill effluent
discharge site. The strains were continuously enriched in mineral salts medium supplemented with PCP as sole source of carbon
and energy. One of the acclimated strains with relatively high PCP degradation capability was selected and characterized in
this study. Based on morphology, biochemical tests, 16S rDNA sequence analysis and phylogenetic characteristics, the strains
showed greatest similarity with Acinetobacter spp. The strain was identified as Acinetobacter sp. ISTPCP-3. The physiological characteristics and optimum growth conditions of the bacterial strain were investigated.
The results of optimum growth temperature revealed that it was a mesophile. The optimum growth temperature for the strain
was 30°C. The preferential initial pH for the strain was ranging at 6.5–7.5, the optimum pH was 7. The bacterium was able
to tolerate and degrade PCP up to a concentration of 200 mg/l. Increase in PCP concentration had a negative effect on biodegradation
rate and PCP concentration above 250 mg/l was inhibitory to its growth. Acinetobacter sp. ISTPCP-3 was able to utilize PCP through an oxidative route with ortho ring-cleavage with the formation of 2,3,5,6-tetrachlorohydroquinone and 2-chloro-1,4-benzenediol, identified using gas chromatograph–mass
spectrometric (GC–MS) analysis. The degradation pathway followed by isolated bacterium is different from previously characterized
pathway. |
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Keywords: | Acinetobacter sp ISTPCP-3 Biodegradation Characterization Gas chromatograph– mass spectrometer Pentachlorophenol |
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