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Quantitation of intracellular polychlorobiphenyls during growth of Rhodotorula glutinis |
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| Authors: | P. Oudin J.A. Toth R. Bonaly C. Balogh |
| Affiliation: | (1) UMR Université-CNRS 7564-LCPE: Biochimie Microbienne Faculté de Pharmacie 5, rue A. Lebrun, F-54000 Nancy, France;(2) Ecological Department, State University of Debrecen KLTE Pf71, H- 4010 Debrecen, Hungary;(3) Regional Inspectory of the Hungarian Ministry of Environment, Piac utca 9b Pf27, H-4001 Debrecen, Hungary |
| Abstract: | A quantitative method to assay accumulated polychlorobiphenyls (PCB) in Rhodotorula glutinis is described which avoids any interference of extracellular adsorbed compounds. This method is based on a prior appropriate treatment of the cells before the extraction of derivatives by supercritical CO2 and GC analysis. The prior treatment consists in a double fixation of the samples with glutaraldehyde and osmium tetroxyde. After this fixation process the elimination of the extracellular PCBs can be achieved by washing the fixed yeast cells with organic solvents. |
| Keywords: | bioaccumulation PCB yeast |
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