Rabies virus infection of primary neuronal cultures and adult mice: failure to demonstrate evidence of excitotoxicity |
| |
Authors: | Weli Simon C Scott Courtney A Ward Christopher A Jackson Alan C |
| |
Institution: | Kingston General Hospital, 76 Stuart Street, Connell 725, Kingston, ON, Canada K7L 2V7. |
| |
Abstract: | Cultures derived from the cerebral cortices and hippocampi of 17-day-old mouse fetuses infected with the CVS strain of rabies virus showed loss of trypan blue exclusion, morphological apoptotic features, and activated caspase 3 expression, indicating apoptosis. The NMDA (N-methyl-D-aspartate acid) antagonists ketamine (125 microM) and MK-801 (60 microM) were found to have no significant neuroprotective effect on CVS-infected neurons, while the caspase inhibitor Ac-Asp-Glu-Val aspartic acid aldehyde (25 microM) exerted a marked neuroprotective effect. Glutamate-stimulated increases in levels of intracellular calcium were reduced in CVS-infected hippocampal neurons. Ketamine (120 mg/kg of body weight/day intraperitoneally) given to CVS-infected adult mice produced no beneficial effects. We have found no supportive evidence that excitotoxicity plays an important role in rabies virus infection. |
| |
Keywords: | |
本文献已被 PubMed 等数据库收录! |
|