Multiple Forms of the Constitutive Wheat Cinnamyl Alcohol Dehydrogenase |
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Authors: | PILLONEL, CHRISTIAN HUNZIKER, PETER BINDER, ANDRES |
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Abstract: | Three cinnamyl alcohol dehydrogenase (CAD) isoenzymes were separatedfrom etiolated wheat seedlings (Triticum aestivum L.) and examinedby native gel electrophoresis. Two of these enzymes (CAD-1 andCAD-2) were purified to apparent homogeneity. They exhibiteda marked difference in substrate affinity. On sodium dodecylsulphate-acrylamide gel the isolated isoenzymes showed onlyone protein band each with an Mr 45000 and 40000 daltons, respectively,whereas on native gel two bands were identified for each protein.Isoenzymes from a variety of diploid, tetraploid, and hexaploidwheats were compared. The results indicated that the CAD polymorphismcould be genetically determined. Key words: Cinnamyl alcohol dehydrogenase, lignin, Triticum aestivum L |
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