Expression of fetal-type intermediate filaments by 17-day-old rat Sertoli cells cultured on reconstituted basement membrane |
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Authors: | Florian Guillou Catherine Monet-Kuntz Isabelle Fontaine Jacques E Flechon |
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Institution: | (1) Unité endocrinologie de la gamétogénèse , INRA, station de Physiologie de la Reproduction, CNRS URA 1291, Nouzilly, France;(2) Biologie cellulaire, INRA, Bâtiment des biotechnologies, Jouy-en-Josas, France;(3) Unité endocrinologie de la gamétogénèse , INRA, Station de Physiologie de la Reproduction, CNRS URA 1291, F-37380 Nouzilly, France |
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Abstract: | Summary The expression of cytokeratin- and vimentin-type intermediate filaments was studied by means of immunohistochemistry in Sertoli cells cultured on two types of reconstituted basement membrane in two-compartment culture chambers. In situ, the Sertoli cells of 17-day-old rats contained only vimentin intermediate filaments. During culture, a gradual reorganization of intermediate filaments accompanied by an increased cytokeratin immunoreactivity was observed. After 6 days, Sertoli cells contained both cytokeratin and vimentin, and the same cytokeratin type as in fetal and newborn testis was revealed by electrophoresis and immunoblotting. The present study shows that the isolation and culture of Sertoli cells causes, even in an improved culture system qualitative changes in the expression of intermediate filament proteins. |
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Keywords: | Intermediate filaments Cytokeratin Vimentin Sertoli cells Testis Basement membrane Rat (Wistar) |
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