Long-term culture of human endothelial cells |
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Authors: | Portia B Gordon Ira I Sussman Victor B Hatcher |
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Institution: | (1) Departments of Biochemistry and Medicine, Albert Einstein College of Medicine, Montefiore Medical Center, 10467 Bronx, New York |
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Abstract: | Summary Human umbilical vein endothelial cells can be grown in vitro for 28 passages (CPDL 58) in Medium 199 supplemented with newborn
bovine serum and a partially purified growth factor derived from bovine brain. Newborn bovine serum is superior to fetal bovine
serum for the proliferation of human umbilical vein endothelial cells seeded at low density in the presence of the growth
factor. The endothelial cells, which can be passaged every 7 to 10 d at a 1-to-5 split ratio, retain their morphological and
biochemical characteristics. The proliferation of cells seeded at low density (103/cm2) is proportional to the concentration of the growth factor present in the medium. The growth factor, which has an isoelectric
point between 5.0 and 5.5, can support cell proliferation at reduced serum concentrations; half-maximal growth is achieved
in medium containing the growth factor and 3% serum. The brain endothelial cell growth factor does not stimulate DNA synthesis
significantly in cultures of human skin fibroblasts.
This research was supported by grants from the U.S. Public Health Service (AG 01732, HL 16387, and HL 07080), the Cystic Fibrosis
Foundation, and the New York and American Heart Associations.
Victor B. Hatcher is an Established Fellow of the New York Heart Association and a recipient of the Ann Weinberg Cystic Fibrosis
Research Scholarship Award. |
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Keywords: | human endothelial cells growth factor |
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