Cloning and characterization of a gene encoding the endopolygalacturonase of Penicillium griseoroseum |
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Authors: | A.O.B. Ribon J.L.C. Coelho E.G. de Barros E.F. Araújo |
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Affiliation: | (1) Departamento de Microbiologia, Universidade Federal de Viçosa, Viçosa, MG, Brazil;(2) Departamento de Biologia Geral, Universidade Federal de Viçosa, Viçosa, MG, 36571-000, Brazil |
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Abstract: | A conserved region of a polygalacturonase (PG) gene from Penicillium griseoroseum was PCR amplified and used to screen a genomic library from this fungus. The nucleotide sequence of the isolated clone (pggI) consisted of 1497 bp, including a coding region of 1251 bp. This region potentially encodes a protein of 376 amino acids, and is interrupted by two introns. Extensive homology was observed between this protein and several fungal endopolygalacturonases. DNA hybridization analyses revealed that there is a low copy number of pggI in the P. griseoroseum genome, probably one or two copies. |
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Keywords: | filamentous fungi gene cloning pectinase sequence homology |
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