电镜原位杂交观察小鼠中期染色体内RNA分布特点及其来源

本文用RNase-gold标记法处理小鼠中期染色体切片,发现染色单体切面内部及周边有大量较均匀分布的RNA。以玉米18 s rRNA的cDNA及水稻5srRNA的DNA为探针,经电镜原位杂交观察到了小鼠18srRNA和5srRNA均在中期染色单体切面内部及边缘有分布,从而首次证明小鼠中期染色体RNA除来源于核仁外,还可以来源于核基质中的5srRNA,但这并非仅有的两种来源。

STUDY ON THE DISTRIBUTION AND ORIGINS OF RNA IN THE METAPHASE CHROMOSOMES OF MOUSE BY IN SITU HYBRIDIZATION AT ELECTRON MICROSCOPIC LEVEL

The RNAs in the metaphase chromosomes of mouse are located by use of RN-ase-gold labelling. The result shows that a lot of RNAs distribute uniformly in the inner and peripheral regions of the chromosome sections. Moreover, the distributions of 18 s rRNA and 5 s rRNA which are studied under the electron microscope by in situ hybridization indicate that 18s rRNA and 5 s rRNA are two among the several origins of the inner RNAs and peripheral RNAs in mouse metaphase chromosomes;