The complete amino acid sequence of a subtilisin inhibitor from adzuki beans (Vigna angularis) |
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Authors: | H Nozawa H Yamagata Y Aizono M Yoshikawa T Iwasaki |
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Affiliation: | Department of Biological Fundamentals, Graduate School of Science and Technology, Kobe University, Hyogo. |
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Abstract: | The complete amino acid sequence of a major molecular form of subtilisin inhibitor from adzuki beans (Vigna angularis) was established by manual analysis using 4-N,N-dimethylaminoazobenzene-4'-isothiocyanate (DABITC). Sequencing was performed on the peptides which were derived by digesting the inhibitor with lysyl-endopeptidase and Staphylococcus aureus V8-protease. The inhibitor consisted of 92 amino acid residues and the molecular weight was calculated to be 10,800. A minor form of subtilisin inhibitor was found, which lacked the amino-terminal 19 residues of the major one. Comparison of amino acid sequences revealed that the adzuki bean subtilisin inhibitors were 29-68% homologous in sequence to the inhibitors of so-called "potato inhibitor I family." |
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