Physical mapping of the 18S-25S and 5S ribosomal RNA genes in diploid bananas |
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Authors: | M Doleželová M Valárik R Swennen JP Horry J Doležel |
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Institution: | (1) Academy of Sciences of the Czech Republic, Sokolovská 6, Institute of Experimental Botany, CZ-77200 Olomouc, Czech Republic;(2) Institute of Experimental Botany, Sokolovská 6, De Montfort University Norman Borlaug Centre for Plant Science, CZ-77200 Olomouc, Czech Republic;(3) Laboratory of Tropical Crop Improvement, Katholieke Universiteit Leuven, Heverlee, Belgium;(4) International Network for the Improvement of Banana and Plantain, Montpellier, France |
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Abstract: | Fluorescent in situ hybridisation (FISH) was used to determine the number and distribution of the 18S-25S and 5S rDNA sites
on mitotic chromosomes of 6 wild and 2 edible diploid (2n=22) accessions belonging to the two banana species, Musa acuminata
and M. balbisiana. FISH with the 18S-25S probe resulted in signals on one pair of chromosomes, the position of signals corresponded
to the secondary constriction at the end of a short arm. The intensity of labelling was different between the homologues and
the larger site corresponded to a larger secondary constriction. This labelling pattern was observed consistently in all genotypes.
On the other hand, differences in the number of 5S sites were observed between the accessions. While in some of the wild seeded
species, the 5S rDNA was localised on two pairs of chromosomes, hybridisation signals appeared on three pairs of chromosomes
in other wild accessions. Quite unexpectedly, only five sites of 5S rDNA were reproducibly observed in the two vegetatively
propagated diploid edible cultivars, Pisang Mas and Niyarma Yik, evidence for structural heterozygosity. A dual colour FISH
showed that in all accessions, the satellite chromosomes carrying the 18S-25S loci did not carry the 5S loci. The results
demonstrate that molecular cytogenetics can be applied to Musa and that physical cytogenetic maps can be generated.
This revised version was published online in July 2006 with corrections to the Cover Date. |
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Keywords: | Musa acuminata Musa balbisiana fluorescent in situ hybridisation FISH |
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