Salmonella typhimurium acrB-like gene: identification and role in resistance to biliary salts and detergents and in murine infection |
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Authors: | Fabrice J.C Lacroix,Axel Cloeckaert,Olivier Gré pinet,Catherine Pinault,Michel Y Popoff,Hervé Waxin,Pierre Pardon |
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Affiliation: | INRA, Centre de Tours-Nouzilly, Pathologie Infectieuse et Immunologie, 37380 Nouzilly, France; Institut Pasteur, Unitédes Entérobactéries, UnitéINSERM 389, 28 Rue du Docteur Roux, 75724 Paris Cedex 15, France |
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Abstract: | Abstract Salmonella serotype typhimurium transpositional mutants altered in resistance to biliary salts and detergents were isolated previously. We have characterized further the LX1054 mutant strain, the most sensitive of them. The chromosomal DNA segment flanking transposon insertion was cloned and sequenced. The highest level of identity was found for the acrB (formerly acrE ) gene of Escherichia coli , a gene encoding a drug efflux pump of the Acr family. LX1054 exhibited a reduced capacity to colonize the intestinal tract. After passages in mice, the mutant strain lost the sensitive phenotype. In vitro, a resumption of growth appeared after 17 h of culture in medium with cholate or other tested biological or chemical detergents. Then, the acquired resistant phenotype seemed stable. The data suggested a role of S. typhimurium acrB -like gene in resistance to biliary salts and detergents and in mice intestinal colonization. However, the local and transient sensitivity observed in vivo, and the in vitro adaptations suggest that several detergent-resistance mechanisms operate in S. typhimurium . |
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Keywords: | Salmonella typhimurium Biliary salt sensitive mutant Detergent resistance Murine infection acr gene |
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