Biogenic amines in cultured neuroblastoma and astrocytoma cells |
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Authors: | R Narotzky W Bondareff |
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Institution: | From the Northwestern University Medical School, Department of Anatomy, Chicago, Illinois 60611 |
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Abstract: | The presence of biogenic amines in cultured cells of mouse neuroblastoma C-1300 (clone NB-2a) was suggested by fluorescence-microscope histochemistry. Incubation in media containing L-14C]tyrosine and L-14C]tryptophan for 24 h, followed by high-voltage electrophoresis, radiochromatogram scanning, and scintillation counting, confirmed the presence of 14C]dopamine, 14C]norepinephrine, 14C]epinephrine, 14C]serotonin, 14C]tyramine, and 14C]octopamine. Dopamine, norepinephrine, epinephrine, and serotonin were demonstrated spectrophotofluorometrically in concentrations, expressed as micrograms amine per milligram protein, of 1.19, 0.027, 0.038, and 0.148, respectively, for cells in a stationary growth phase. Fluorescence-microscope histochemistry also suggested the presence of biogenic amines in cultured astrocytoma cells (cell line C6). Spectrophotofluorometric assay of cells in a stationary growth phase demonstrated intracellular dopamine, norepinephrine, epinephrine, and serotonin in concentrations significantly lower than those of neuroblastoma cells. |
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