大豆PEBP基因家族的分析

磷脂酰乙醇胺结合蛋白(PEBP,phosphatidyl ethanolamine-binding protein)基因家族在动物、植物和微生物中广泛存在,在控制植物开花和种子休眠中起重要作用。本研究对大豆PEBP基因家族进行了分析,发现了27个大豆PEBP基因的候选序列,其中16个具有完整PEBP结构域的全长序列被认为是大豆Gm PEBP家族基因。Gm PEBP基因分布在9条染色体上,基因结构高度保守。通过系统发生分析,可将大豆Gm PEBP基因家族成员分为FT-like、TFL1-like和MFT-like 3个亚族,并且发现Gm PEBP家族成员数目按照大豆物种特异性的方式进行了扩张。对重复基因的Ks分析表明,绝大多数重复基因主要由5900万年前和1300万年前的大豆基因组复制所致。

Analysis of the PEBP gene family in soybean (Glycine max)

The PEBP gene family encodes proteins with similarity to Phosphatidylethanolamine binding Proteins which found across the animals, plants and microbes. The PEBP genes have important roles in regulation of flowering time and seed dormancy. We presented here a systematic analysis of the PEBP gene family in soybean (Glycine max). At the level of genome-wide screening, we identified 27 PEBP candidate genes from soybean and considered 16 genes with full length of coding sequence as GmPEBP genes. Squence analysis showed the GmPEBP genes had conserved genomic structures and were distributed on nine soybean chromosomes. Genomic structures and chromosome locations were determined for the 16 GmPEBP genes. Phylogenetic analysis grouped the GmPEBP family into three subfamilies, namely FT-like, TFL1-like and MFT-like, and suggested that species-specific expansion contributed to the evolution of this family. Analysis of synonymous substitution rates indicated most duplicated pairs were from the whole genome duplication occurred at 59 and 13 million years ago.