| 大豆MYB转录因子基因GmMYB174的克隆及分子特性分析 |
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| 引用本文： | 魏麦玲,裴丽丽,徐兆师. 大豆MYB转录因子基因GmMYB174的克隆及分子特性分析[J]. 植物遗传资源学报, 2015, 16(1): 94-99 |
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| 作者姓名： | 魏麦玲 裴丽丽 徐兆师 |
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| 作者单位： | 中国农业科学院作物科学研究所 |
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| 基金项目： | 转基因生物新品种培育科技重大专项(2014ZX0800203B);陕西省科技计划项目(2014K02-04-05) |
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| 摘 要： | 克隆获得了大豆转录因子MYB基因GmMYB174,该基因序列全长1086 bp,编码361个氨基酸,属于MYB-related家族。生物信息学分析表明大豆GmMYB174与番茄、苜蓿、葡萄等物种同源性较高,序列分析表明包含2个保守的Trp(W)位点和1个保守基序SHAQKFF。亚细胞定位结果显示GmMYB174定位于细胞核中;组织表达量显示GmMYB174在多个组织均有表达,其中在上胚轴中表达量最高。启动子元件分析表明GmMYB174包含ABRE、MYB、MYC、LTRE、GT-1等逆境胁迫应答元件。实时荧光定量PCR分析表明Gm MYB174在干旱、盐、ABA处理下均有响应。因此,GmMYB174可能参与多种胁迫应答途径。
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| 关 键 词： | 大豆 MYB转录因子 非生物胁迫 响应机制 亚细胞定位 |
| 收稿时间： | 2014-06-21 |
| 修稿时间： | 2014-08-25 |
Isolation and molecular characteristics analysis of soybean transcription factor gene MYB174 |
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| Xu Zhao-Shi. Isolation and molecular characteristics analysis of soybean transcription factor gene MYB174[J]. Journal of Plant Genetic Resources, 2015, 16(1): 94-99 |
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| Authors: | Xu Zhao-Shi |
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| Affiliation: | WEI Mai-ling;PEI Li-li;LIU Jia-ming;MIN Dong-hong;CHEN Ming;LI Lian-cheng;MA You-zhi;XU Zhao-shi;ZHANG Xiao-hong;College of Life Sciences,Northwest A&F University/State Key Laboratory of Crop Stress Biology for Arid Areas;Institute of Crop Sciences,Chinese Academy of Agricultural Sciences(CAAS)/National Key Facility for Crop Gene Resources and Genetic Improvement/Key Laboratory of Biology and Genetic Improvement of Triticeae Crops,Ministry of Agriculture; |
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| Abstract: | The full sequence of GmMYB174 was isolated from soybean, including 1086 bp and encoding 361 amino acids. GmMYB174 belonged to MYB-related family. Bioinformatics analysis indicate GmMYB174 share a high similar with tomato, alfalfa and peanut, containing 2 conserved locus and a motif of SHAQKFF. GmMYB174 located in nucleus. The expression predication of tissue show that GmMYB174 expressed in many tissues and peaked in epicotyl. Promoter analysis indicate that the region of GmMYB174 promoter contain ABRE, MYB, MYC, LTRE and GT-1. Q-RT-PCR shows that GmMYB174 involved in various abiotic stresses. GmMYB174 maybe participated in multi-environmental stress responses. |
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